Enzyme Catalysis

We seek the general principles that govern enzyme catalysis by understanding the unique mechanism and action of individual enzymes.

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   Theory

Wb00955_.gif (255 bytes)    Contribution from Protein Conformation Transition

Wb00955_.gif (255 bytes)    Mechanisms and Free Energies of Catalysis

Wb00955_.gif (255 bytes)    Dynamics and Tunneling in Catalysis

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   Illustrative Examples (an incomplete list)

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Wb00955_.gif (255 bytes)    Protein Tyrosine Phosphatases

Wb00955_.gif (255 bytes)    Cathepsin K

Wb00955_.gif (255 bytes)    Phosphotriesterase (PTE)

Wb00955_.gif (255 bytes)    Alanine Racemase (Carbon Acidity)

              Alanine Racemase (Mechanism)

Wb00955_.gif (255 bytes)    Enolase

Wb00955_.gif (255 bytes)    Haloalkane Dehalogenase

              Dynamics and Mechanism

Wb00955_.gif (255 bytes)    Decarboxylases

              C&E News report on OMP Decarboxylase

Wb00955_.gif (255 bytes)    4-Oxalocrotonate Tautomerase

Wb00955_.gif (255 bytes)    Hairpin Ribozyme

Wb00955_.gif (255 bytes)    Nitroalkane Oxidase

Wb00955_.gif (255 bytes)    Dopa Decarboxylase

Wb00955_.gif (255 bytes)    Acutolysin

Wb00955_.gif (255 bytes)    Aristolochene Synthase

Wb00955_.gif (255 bytes)    Dihydrofolate Reductase (DHFR)

              Temperature dependence KIE

              Reaction Path and Mechanism

              Nature of Transition State

              KIE in Hyperthermophilic DHFR

Wb00955_.gif (255 bytes)    Squalene Cyclase

Wb00955_.gif (255 bytes)    4-Chlorobenzoyl-CoA dehalogenase

Wb00955_.gif (255 bytes)    Acyl-CoA Dehydrogenase

              Mechanism, KIE, and Reaction Path

              Redox Potential

Wb00955_.gif (255 bytes)    Liver alcohol dehydrogenase

              Tunneling

Wb00955_.gif (255 bytes)    Xylose Isomerase

Wb00955_.gif (255 bytes)    Carbonic Anhydrase

Wb00955_.gif (255 bytes)    HIV-1 Protease

 

 

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Top view of ODCase beta-barrel dimer

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Side view of ODCase

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Electron density map at the ODCase active site

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